منابع مشابه
Photoactivated covalent binding of [3H]bilirubin to human serum albumin.
A one-step procedure has been developed for the preparation of [3H]bilirubin IX-alpha in good yield from unlabelled bilirubin. Irradiation of an aqueous solution of [3H]bilirubin IX-alpha in the presence of human serum albumin results in the covalent attachment of the bilirubin to the protein. Preliminary degradation studies have been carried out to locate the site of attachment of the bilirubi...
متن کاملBinding of [3H]bilirubin to albumin and plasma membrane vesicles.
Arterial oxygen partial pressures reduce the insulin-dependent induction of the perivenously located glucokinase in rat hepatocyte cultures : mimicry of arterial oxygen pressures by H 2 O 2 Proteins Protease digestion studies of an equilibrium intermediate in the unfolding of creatine kinase Mucus glycoproteins in bovine trachea : identification of the major mucin populations in respiratory sec...
متن کاملAltered labelling of the cell surface and intracellular organelles with [3H]mannose in enucleated amoebae.
The production, transport, and disposition of material labelled with [3H]mannose were studied in microsurgically enucleated and control amoebae. Cells were injected with the precursor and samples were prepared for electron-microscope radioautography at intervals, up to 24 h later. Control cells showed heavy labelling of the rough endoplasmic reticulum and the Golgi apparatus at early intervals ...
متن کاملLabelling of human resting lymphocytes by continuous infusion of [3H]thymidine. I. Characterization of cytoplasmic label.
After continuous 3H-TdR infusion in vivo or incubation with 3H-TdR in vitro human blood lymphocytes were examined by light-microscopic and electron-microscopic autoradiography. Using relatively long autoradiographic exposure times (50--300 days) not only nuclear but also cytoplasmic labelling was visualized, the cytoplasmic label being present in up to 96% of the cells. The cytoplasmic label wa...
متن کاملEffects of vitamin D3 on in vivo labelling of chick skeletal muscle proteins with [3H]leucine.
The effects of the administration of a single oral dose of vitamin D3 to rachitic chicks on the in vivo incorporation of [3H]leucine to proteins of skeletal muscle subcellular fractions was studied. A significant stimulation (50%) of labelling of mitochondrial proteins could be seen. The sterol affected the labelling of sarcoplasmic reticulum (20%) and contractile proteins (10%) to a lesser ext...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1976
ISSN: 0264-6021
DOI: 10.1042/bj1570511